Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Protocol for minimizing necrotic core formation in iPSC-derived cortical organoids.

STAR protocols·2026

Same author

Neuroimmune mechanisms in 16p11.2 deletion Syndrome: Emerging Roles for complement signaling.

Brain, behavior, and immunity·2026

Same author

The persistence of potential: The life of Sir John B. Gurdon.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

In-Vivo 6D Heart Motion Analysis for Emerging Self-Powered Cardiac Implants.

Annals of biomedical engineering·2026

Same author

Untangling the Molecular Mechanisms Contributing to Autism Spectrum Disorder Using Stem Cells.

Autism research : official journal of the International Society for Autism Research·2025

Same author

Cellular mechanisms of early brain overgrowth in autistic children: elevated levels of GPX4 and resistance to ferroptosis.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: May 12, 2026

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Published on: July 17, 2019

A simple tool to improve pluripotent stem cell differentiation.

Sundari Chetty¹, Felicia Walton Pagliuca, Christian Honore

¹Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.

|April 16, 2013

Summary

This summary is machine-generated.

Dimethyl sulfoxide (DMSO) treatment enhances human pluripotent stem cell differentiation. This method activates key proteins and improves cell cycle progression, leading to better directed differentiation into various cell types.

More Related Videos

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation

Published on: October 4, 2024

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

Related Experiment Videos

Last Updated: May 12, 2026

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Published on: July 17, 2019

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation

Published on: October 4, 2024

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

Area of Science:

Stem cell biology
Developmental biology
Cellular differentiation

Background:

Human pluripotent stem cells (hPSCs) have limitations in directed differentiation.
Controlling differentiation propensities is crucial for regenerative medicine and disease modeling.

Purpose of the Study:

To present a method for overcoming differentiation restrictions in hPSCs.
To improve directed differentiation of hPSCs into multiple lineages.

Main Methods:

Culturing hPSCs in dimethyl sulfoxide (DMSO).
Assessing retinoblastoma protein activation.
Analyzing cell cycle phase distribution.
Evaluating differentiation into multiple lineages across diverse hPSC lines.

Main Results:

DMSO treatment activates the retinoblastoma protein.
Increased proportion of cells in the early G1 phase of the cell cycle.
Improved directed differentiation into multiple lineages in over 25 hPSC lines.
Enhanced differentiation into terminal cell types.

Conclusions:

DMSO is an effective agent for improving hPSC differentiation.
This method offers a strategy to enhance the utility of hPSCs for various applications.
The findings have implications for stem cell-based therapies and research.