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Quantitative mouse renal perfusion using arterial spin labeling.

Reshmi Rajendran¹, Si Kang Lew, Cai Xian Yong

¹Magnetic Resonance Imaging Group, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore.

NMR in Biomedicine

|April 18, 2013

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Arterial spin labeling MRI successfully quantified mouse renal perfusion, overcoming previous challenges. This noninvasive technique shows promise for studying kidney function and disease models.

Area of Science:

Biomedical Imaging
Renal Physiology
Magnetic Resonance Imaging

Background:

Accurate renal perfusion information is crucial for diagnosing and prognosing kidney function.
Gadolinium-based contrast agents have limitations for renal perfusion quantification due to glomerular filtration and safety concerns in patients with kidney dysfunction.
Arterial spin labeling (ASL) MRI offers a noninvasive method for perfusion quantification but faces challenges in mouse models due to low sensitivity and artifacts.

Purpose of the Study:

To investigate the feasibility and application of ASL MRI for quantifying renal perfusion in mice.
To optimize ASL MRI parameters for high-quality mouse renal perfusion imaging.
To assess the impact of oxygen and carbogen breathing on mouse renal perfusion and T1 values.

Main Methods:

Keywords:

MRI arterial spin labeling carbogen flow-sensitive alternating inversion recovery (FAIR)kidney perfusion translational

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Mouse renal perfusion was studied using flow-sensitive alternating inversion recovery (FAIR) ASL MRI at 7 Tesla.
Respiratory, susceptibility, and fat artifacts were controlled using triggering, high-order shimming, and water excitation, respectively, with spin-echo echo-planar imaging.
Perfusion measurements were compared between 100% oxygen and carbogen (95% O2/5% CO2) breathing conditions.

Main Results:

High-quality renal perfusion images were obtained in mice.
Renal cortical perfusion was significantly higher than medullary perfusion.
Cortical perfusion increased from 397 ± 36 to 476 ± 73 mL/100g/min under carbogen.
Medullary perfusion increased from 166 ± 41 to 203 ± 40 mL/100g/min under carbogen.
T1 relaxation times decreased in both cortex and medulla under carbogen compared to 100% oxygen.

Conclusions:

ASL MRI, particularly with FAIR technique at 7T, is a viable noninvasive method for quantifying mouse renal perfusion.
The study demonstrated significant differences in cortical and medullary perfusion and their responses to gas challenges.
This technique holds potential for advancing research in mouse models of renal diseases.