Related Experiment Video
Updated: May 12, 2026

09:31
Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging
Published on: March 27, 2020
A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence
Dorothea Pinotsi1, Alexander K Buell, Christopher M Dobson
1Department of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge, CB2 3RA, UK.
Chembiochem : a European Journal of Chemical Biology
|April 18, 2013
Abstract:
Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein (blue curve) but not when α-synuclein seeds are incubated with β-synuclein monomeric protein (black curve), thus showing that no seeded growth occurred in this case.
