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Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary

Terence G Henares1, Yuta Uenoyama, Yuto Nogawa

  • 1Graduate School of Engineering, Osaka Prefecture University, Sakai City, Osaka 599-8531, Japan.

The Analyst
|April 19, 2013
PubMed

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Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.