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A simplified radiometabolite analysis procedure for PET radioligands using a solid phase extraction with micellar
Ryuji Nakao1, Christer Halldin
1Department of Clinical Neuroscience, Center for Psychiatric Research, Karolinska Institutet, Stockholm, Sweden. ryuji.nakao@ki.se
Nuclear Medicine and Biology
|April 23, 2013
Summary
A new solid phase extraction method using a micellar medium allows for rapid and direct determination of positron emission tomography (PET) radioligands in plasma, simplifying analysis and improving throughput for quantitative imaging studies.
Area of Science:
- Analytical Chemistry
- Radiochemistry
- Biomedical Imaging
Background:
- Positron emission tomography (PET) imaging requires accurate quantification of radioligands.
- Metabolism of radioligands leads to the formation of radioactive metabolites, complicating analysis.
- Existing methods for radioligand and metabolite determination can be time-consuming and require extensive sample preparation.
Purpose of the Study:
- To develop a simple, high-speed method for direct determination of PET radioligands in plasma.
- To achieve efficient separation of parent radioligands from their radioactive metabolites.
- To enhance the throughput for analyzing plasma samples in quantitative PET studies.
Main Methods:
- Development of a solid phase extraction (SPE) method utilizing a micellar medium.
- Use of an anionic surfactant (sodium dodecyl sulphate) in the micellar eluent.
- Direct analysis of plasma samples from human or monkey during PET measurements.
Main Results:
- Achieved excellent separation of PET radioligands from metabolites with a 1-minute run-time.
- Demonstrated successful application in studying metabolism of (11)C-labelled radioligands.
- Showed strong correlation between micellar SPE results and radio-LC, with high throughput (up to 13 samples/study).
Conclusions:
- Micellar solid phase extraction is a fast, sensitive, and user-friendly method.
- Offers an attractive alternative for determining the relative composition of PET radioligands in plasma.
- Facilitates accurate metabolite-corrected input function estimation for quantitative PET imaging.
