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Updated: May 12, 2026
13:46
An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
Published on: July 11, 2020
BDNF-induced local protein synthesis and synaptic plasticity.
Graciano Leal1, Diogo Comprido, Carlos B Duarte
1CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal; Department of Life Sciences, University of Coimbra, 3004-517 Coimbra, Portugal.
Neuropharmacology
|April 23, 2013
Summary
Brain-derived neurotrophic factor (BDNF) regulates synaptic plasticity and memory formation by influencing mRNA translation and protein synthesis. This neurotrophin
Area of Science:
- Neuroscience
- Molecular Biology
- Synaptic Plasticity
Background:
- Brain-derived neurotrophic factor (BDNF) is crucial for synaptic transmission and long-term potentiation (LTP).
- BDNF's effects are mediated by tropomyosin-related kinase B (TrkB) receptors and downstream signaling pathways (Ras/ERK, PI3K/Akt, PLC-γ).
- BDNF plays a role in learning and memory formation, particularly in the hippocampus.
Purpose of the Study:
- To review recent advancements in understanding BDNF's mechanisms regulating the synaptic proteome.
- To explore BDNF's role in short- and long-term synaptic plasticity.
- To highlight BDNF's influence on learning and memory.
Main Methods:
- Literature review focusing on recent studies.
- Analysis of molecular mechanisms involving mRNA transport, translation, and miRNA regulation.
- Examination of BDNF's impact on synaptic protein synthesis and transcription.
Main Results:
- BDNF regulates mRNA transport and translation at synapses, affecting protein synthesis initiation and elongation.
- BDNF influences specific microRNAs (miRNAs) involved in synaptic regulation.
- BDNF-mediated transcription regulation contributes to long-term synaptic proteome changes.
Conclusions:
- BDNF is a key regulator of synaptic proteome dynamics through pre- and post-synaptic mechanisms.
- Understanding BDNF's role in synaptic plasticity is vital for comprehending learning and memory processes.
- This review synthesizes current knowledge on BDNF's multifaceted regulation of synaptic function.
Keywords:
4EBPA2READFASArcBDNFBrain-derived neurotrophic factor (BDNF)CPECPEBCREBCYFIP1Ca(2+)- and calmodulin-dependent protein kinase IICa(2+)- and calmodulin-dependent protein kinase kinaseCaMKIICaMKKDGE-LTPEJCFMRPGIPC1GRIP1HFSIEGIP3IRESL-LTPLIM domain kinase 1LIMK1LTPLong-term potentiation (LTP)MAPMAPK and ERK kinase, type 1/2MEK1/2MSK1NT-4P-bodiesPAKPDZ (postsynaptic density-95/Discs large/zona occludens-1) domain-containing adaptor protein, type 1PH domainPI3-KPICK1PLC-γPSDPSD95PSFPum2RISCRNA processing bodiesRNA transportRNA-induced silencing complexRab3a interacting molecule 1αRim1αSAM68SAP97Src-associated in mitosis of 68 kDaSynaptic plasticityTORCTRPC3TranslationTrkBZBP1actin-depolymerizing factoractivity-regulated cytoskeleton-associated proteinantisensebrain-derived neurotrophic factorcAMP-response element-binding proteincytoplasmic Fmr-interacting protein 1cytoplasmic polyadenylation elementcytoplasmic polyadenylation element-binding proteindentate gyruseEFeIFeIF4E-binding proteinearly-LTPeukaryotic elongation factoreukaryotic initiation factorexon junction complexfragile X mental retardation proteinglutamate receptor-interacting protein 1heterogeneous nuclear ribonucleoproteinheterogeneous nuclear ribonucleoprotein (hnRNP) A2 response elementhigh-frequency stimulationhnRNPimmediate-early genesinositol 1,4,5-trisphosphateinternal ribosomal entry sitelate-LTPlong-term potentiationmRNPsmTORmammalian target of rapamycinmessenger ribonucleoprotein complexesmicrotubule-associated proteinmitogen- and stress-activated kinase 1neurotrophin-4p21-activated kinasephosphatidylinositol 3-kinasephospholipase C-γpleckstrin homology domainpolypyrimidine tract binding protein-associated splicing factorpostsynaptic densitypostsynaptic density protein 95protein interacting with C kinase 1pumilio2synapse-associated protein 97target of rapamycin (TOR) complextransient receptor-potential cation channel subfamily C (TRPC), type 3tropomyosin-related kinase Bzipcode-binding protein 1More Related Videos
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