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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

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BLAST output visualization in the new sequencing era.

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Briefings in Bioinformatics

|April 23, 2013

Summary

This summary is machine-generated.

New bioinformatics tools are needed to handle large Basic Local Alignment Search Tool (BLAST) outputs, especially for biologists without extensive computational training. This review categorizes and evaluates freely available software for processing and visualizing BLAST results.

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Area of Science:

Bioinformatics
Computational Biology
Genomics

Background:

The Basic Local Alignment Search Tool (BLAST) is a fundamental bioinformatics algorithm with widespread use in biological research.
Advancements in sequencing technologies and expanding genomic databases lead to increasingly large BLAST outputs, often exceeding manual processing capabilities.
A growing number of biologists entering the field lack specialized bioinformatics expertise, creating a demand for accessible data analysis tools.

Purpose of the Study:

To review and categorize freely available software designed for processing and visualizing Basic Local Alignment Search Tool (BLAST) output.
To evaluate these tools based on user-friendliness, analytical functionalities, and capacity for high-throughput data analysis.
To address the need for accessible solutions for biologists managing large-scale BLAST results.

Main Methods:

Categorization of software into distinct functional groups: BLAST output interpreters, environments, parsers, and specialized tools.
Evaluation of each tool's usability, feature set for data analysis, and performance with large datasets.
Literature review and assessment of existing bioinformatics software for BLAST output management.

Main Results:

Identification and classification of various freely available programs for handling BLAST outputs.
Assessment of the strengths and weaknesses of different tool categories concerning user experience and analytical power.
Highlighting tools suitable for both novice users and high-throughput research environments.

Conclusions:

User-friendly software solutions are crucial for enabling biologists to effectively analyze large BLAST outputs.
The reviewed tools offer diverse capabilities, catering to different user needs and research scales.
Continued development of accessible bioinformatics tools is essential for advancing biological discovery.