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Updated: May 12, 2026

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
Published on: July 9, 2016
Proteomic analysis of brain mitochondrial proteome and mitochondrial complexes
Ana Lopez-Campistrous1, Carlos Fernandez-Patron
1Department of Biochemistry, Institute for Biomolecular Design, University of Alberta, Edmonton, AB, Canada.
None:
We describe various complementary techniques to achieve multidimensional mitochondrial proteome fractionation and analysis. Previously described methods for 2D-DIGE/mass spectrometry and 1D-SDS-PAGE/Western techniques and protein complex analysis by BN-PAGE/Western and sucrose gradient ultracentrifugation/SDS-PAGE/mass spectrometry are optimized to characterize the brain mitochondrial proteome. This approach allows for a comprehensive identification of mitochondrial proteomic differences between health and disease conditions.
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