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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
Published on: August 16, 2012
On-chip cytometry using plasmonic nanoparticle enhanced lensfree holography
Qingshan Wei1, Euan McLeod, Hangfei Qi
1Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
Scientific Reports
|April 24, 2013
Summary
Lensfree on-chip microscopy uses plasmon-resonant nanoparticles to identify cell types. This high-throughput imaging platform offers molecular specificity over a wide field-of-view for rapid specimen analysis.
Area of Science:
- Biomedical imaging
- Nanotechnology
- Computational microscopy
Background:
- Lensfree on-chip microscopy offers large field-of-view and depth-of-field for high-throughput specimen analysis.
- Compact and lightweight imaging architectures are desirable for rapid analysis of large sample volumes.
Purpose of the Study:
- To introduce molecular specificity into high-throughput lensfree on-chip imaging.
- To demonstrate automated cell type recognition using plasmon-enhanced holograms.
Main Methods:
- Utilizing plasmon-resonant metallic nanoparticles for molecular recognition.
- Employing lensfree on-chip imaging to capture plasmon-enhanced holograms.
- Reconstructing holographic data over a large field-of-view (~24 mm²).
Main Results:
- Successful automatic recognition of different cell types.
- Demonstration of molecular specificity integrated with high-throughput imaging.
- Detection and reconstruction of plasmon-enhanced lensfree holograms.
Conclusions:
- Plasmon-resonant nanoparticles can confer molecular specificity to lensfree on-chip microscopy.
- This approach enables automated cell type identification in a high-throughput manner.
- The developed technique is suitable for analyzing large specimen volumes with enhanced specificity.
