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A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
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Brand of dipotassium EDTA vacuum tube as a new source of pre-analytical variability in routine haematology testing

G Lima-Oliveira1, G Lippi, G L Salvagno

  • 1Laboratory of Clinical Biochemistry, Department of Life and Reproduction Sciences, University of Verona, Italy. dr.g.lima.oliveira@gmail.com

British Journal of Biomedical Science
|April 27, 2013
PubMed

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In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
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Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
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