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A rapid and sensitive gas chromatographic analysis of methadone and its primary metabolite
P Kintz1, P Mangin, A A Lugnier
1Institut de Médecine Légale, Strasbourg.
Journal De Toxicologie Clinique Et Experimentale
|January 1, 1990
Abstract:
A method for identification and quantification of methadone and its primary metabolite (1,5-dimethyl-3,3-diphenyl-2-2-ethylidene pyrrolidine) in plasma by GC/NPD is presented. The procedure employs SKF 525 A as the internal standard and requires no derivatization. After a single-step extraction, analysis is achieved in 10 min. The limit of detection was found to be 2 an 3 ng/ml for methadone and its metabolite, respectively. This method appears to be rapid, sensitive and applicable to forensic and clinical toxicological analyses.