Related Experiment Video
Updated: May 11, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Establishment and application of TaqMan real-time RT-PCR for the detection of hepatitis E virus]
Qing-Ling Meng1, Feng Qiu, Li-Ping Shen
1Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
Objective:
To establish a specific TaqMan-based Real-time PCR assay for the detection of hepatitis E virus (HEV).
Methods:
According to the references, primers-probe sets which were located in ORF2, the conservative part of HEV genome were designed and therefore we established a HEV TaqMan real-time RT-PCR assay with great performance of specificity, sensitivity and reproducibility. And then it was used in the detection of HEV RNA in clinical samples.
Results:
The HEV Real-time RT-PCR assay established in this study were able to detect HEV RNA with a detection limit of 10 copies/reaction. When the detection of a same sample was repeated for several times, coefficients of variation (CV) was all less than 1.53%. Our data also suggested that there were 1.87 x 10(6)-8.12 x 10(9) RNA copies in 1 ml of the clinical samples.
Conclusion:
The TaqMan-based Real-time PCR assay established in this study was specific and precise for the rapid detection of HEV RNA. It was applied successfully in the pathogen detection of clinical samples.
More Related Videos
08:36Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
12:32EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Published on: January 16, 2016
Related Concept Videos
02:57Real Time RT-PCR
The real-time quantification of the number of amplified products is...
01:25Hepatitis