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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

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Related Experiment Video

Updated: May 11, 2026

Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model
09:00

Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model

Published on: April 17, 2026

Decoding ubiquitin for mitosis.

Sadek Fournane1, Ksenia Krupina, Charlotte Kleiss

  • 1Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France.

Genes & Cancer
|May 2, 2013
PubMed
Summary

Ubiquitin conjugation ensures cell division accuracy. This review explores how ubiquitin decoders, deubiquitinating enzymes (DUBs), and ubiquitin-binding proteins (UBPs) regulate faithful mitotic progression.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Ubiquitination regulates numerous cellular processes, including mitosis.
  • Faithful cell division requires precise temporal and spatial coordination of mitotic events.
  • Ubiquitin modifications, beyond proteolysis, play crucial roles in ensuring mitotic fidelity.

Purpose of the Study:

  • To review the role of ubiquitin decoders in mitotic regulation.
  • To summarize emerging concepts on deubiquitinating enzymes (DUBs) and ubiquitin-binding proteins (UBPs) in mitosis.
  • To highlight how these factors contribute to the fidelity of mitotic division.

Main Methods:

  • Literature review of existing research on ubiquitination in mitosis.
  • Analysis of the functions of ubiquitin-interacting proteins (decoders).
Keywords:
DUBsUBPsmitosisubiquitin

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Ubiquitin Chain Analysis by Parallel Reaction Monitoring
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Ubiquitin Chain Analysis by Parallel Reaction Monitoring

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Related Experiment Videos

Last Updated: May 11, 2026

Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model
09:00

Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model

Published on: April 17, 2026

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Published on: July 25, 2019

Ubiquitin Chain Analysis by Parallel Reaction Monitoring
08:33

Ubiquitin Chain Analysis by Parallel Reaction Monitoring

Published on: June 17, 2020

  • Synthesis of current knowledge on DUBs and UBPs in mitotic regulation.

    • Main Results:

    • Ubiquitination provides directionality and fidelity to mitosis.
    • Non-proteolytic ubiquitination pathways are critical for mitotic accuracy.
    • Ubiquitin-binding proteins (UBPs) and deubiquitinating enzymes (DUBs) act as crucial "decoders" of ubiquitin signals.
    • These decoders regulate substrate localization and downstream signaling during mitosis.

    Conclusions:

    • Ubiquitin decoders, including DUBs and UBPs, are essential for faithful mitotic progression.
    • Understanding these regulatory mechanisms provides insights into maintaining genomic stability.
    • Further research into ubiquitin signaling pathways in mitosis is warranted.