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Related Experiment Video

Updated: May 11, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

A tuneable array of unique steady-state microfluidic gradients.

Matthew D Estes1, Cedric Hurth, Matthew Barrett

  • 1Center for Applied NanoBioscience and Medicine, Chandler, AZ, USA. matthew.estes@arizona.edu

Physical Chemistry Chemical Physics : PCCP
|May 3, 2013
PubMed
Summary

This study presents an on-chip gradient generator for precise, parallel control of chemical gradients. The device offers tuneable profiles and intensities for diverse microfluidic applications.

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Area of Science:

  • Microfluidics
  • Biotechnology
  • Chemical Engineering

Background:

  • Precise control of chemical gradients is crucial for various biological and chemical applications.
  • Existing methods for gradient generation often lack parallelization and fine-tuneability.

Purpose of the Study:

  • To design, model, fabricate, and characterize a novel on-chip gradient generator.
  • To enable parallel, temporally tuned generation of unique gradients with precise control over intensity and profile.

Main Methods:

  • The device utilizes on-chip flow inlet ratios for gradient magnitude control, eliminating the need for active mixers.
  • A biotin-streptavidin complex forms an on-chip diffusive barrier for orthogonal control of microgradient intensities.
  • An on-chip enzymatic reaction was employed for device characterization, producing tuneable product concentrations.

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A Gradient-generating Microfluidic Device for Cell Biology
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A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
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A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Published on: April 19, 2010

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Last Updated: May 11, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

A Gradient-generating Microfluidic Device for Cell Biology
11:05

A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
09:28

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Published on: April 19, 2010

Main Results:

  • The gradient generator successfully produced exponential, linear, and logarithmic gradient profiles.
  • Steady-state programming of gradient intensities across multiple orders of magnitude was achieved.
  • Two orthogonal mechanisms allowed for independent regulation of microgradient intensities.

Conclusions:

  • The developed on-chip gradient generator offers a versatile platform for parallel gradient generation.
  • The device provides precise and tuneable control over gradient profiles and intensities for diverse applications.
  • This technology advances microfluidic systems for research and development.