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[Screening of atherosclerosis related polypeptide from phage display peptide library]
Zhi Xiao1, Xuwen Zhao, Ming Zhao
1Department of Pathophysiology, Guangdong Key Laboratory for Shock and Microcirculation, Southern Medical University, China. rzxw86@126.com
Objective:
To screen atherosclerosis (AS) related polypeptide from phage display 12-peptide library, and verify the binding activity of selected positive phages and synthetic protein fragment.
Methods:
We collected plasma from AS patients as target for biopanning against phage-displayed 12-peptide library. After 3 rounds of screening, 10 positive phages were picked up and the binding activity was proved by ELISA. Single-stranded DNA of the phages were purified and sequenced, and a similar polypeptide was synthesized to test the binding activity to AS patients plasma.
Results:
Selected phages significantly bound to AS patients' plasma. Five of ten phages had GPRPPSAPNMPL sequence. Corresponding synthetic polypeptide also showed a high binding activity to AS patient plasma.
Conclusion:
AS-related polypeptide can be obtained by phage display, which facilitates the research into the immune mechanism of AS.
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