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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 11, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

A high-throughput antibody-based microarray typing platform.

Andrew Gehring1, Charles Barnett, Ted Chu

  • 1Molecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture-North Atlantic Area-Agricultural Research Service-Eastern Regional Research Center, Wyndmoor, PA 19038, USA. andrew.gehring@ars.usda.gov

Sensors (Basel, Switzerland)
|May 7, 2013
PubMed
Summary
This summary is machine-generated.

A new high-throughput platform uses laser-induced fluorescence to detect and type microbial contaminants in food. While not sufficient for Shiga toxin-producing E. coli serotyping, it shows potential for rapid microbial identification.

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Last Updated: May 11, 2026

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Area of Science:

  • Food Microbiology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Rapid detection of pathogenic microorganisms in food is crucial for public health.
  • Microbial typing aids in outbreak investigations and source tracing.
  • Existing methods often lack the speed and throughput for comprehensive analysis.

Purpose of the Study:

  • To introduce and evaluate a novel high-throughput platform for microbial typing.
  • To assess the platform's effectiveness for serotyping Shiga toxin-producing E. coli (STEC) and E. coli O157:H7.
  • To compare different antibody sets for pathogen capture and detection.

Main Methods:

  • Development of a high-throughput platform using microarrayed multiwell plates.
  • Detection of antibody-captured bacteria via laser-induced fluorescence with SYBR Gold stain.
  • Testing of antibody sets against non-O157 STEC and E. coli O157:H7.

Main Results:

  • The platform demonstrated reasonable performance for STEC antibody sets.
  • Specificity increased at lower concentrations of capture antibodies or bacterial targets.
  • Antibody specificity was insufficient for direct STEC serotyping.

Conclusions:

  • The high-throughput platform shows potential for rapid microbial typing of food isolates within approximately 80 minutes.
  • Optimization of biorecognition elements (antibodies or aptamers) is key for enhanced specificity.
  • The platform can serve as a tool for antibody testing and pathogen detection in food enrichments.