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Updated: May 11, 2026
5-Ethynyl-2'-Deoxyuridine/Phospho-Histone H3 Dual-Labeling Protocol for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells
Published on: May 4, 2021
[Method of selective demonstration of proliferating cells in the structures of developing nervous system based on the
D E Korzhevskiy1, O V Kirik, G V Beznin
1RAMS North-Western Branch Institute of Experimental Medicine, St. Petersburg, Russia
Abstract:
Phosphorylated H3 histone is of interest for the study of proliferative activity of the cells in various tissues. Its detection is used in histopathological tumor diagnosis. The purpose of this study was to develop immunocytochemical protocols for the demonstration of H3 phosphohistone in the cells of the developing nervous system for classical light microscopy and confocal laser microscopy. The protocols presented allow to identify the dividing cells in the structures of developing brain with high selectivity and good reproducibility. Zinc-ethanol-formaldehyde is recommended as an optimal fixative for H3 phosphohistone.
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