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Updated: May 11, 2026
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Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison
Published on: October 20, 2020
Examination of biologically active nanocomplexes by nanoparticle tracking analysis
Nikolai Nikitin1, Ekaterina Trifonova, Olga Karpova
1Department of Virology, Moscow State University, Moscow 119991, Russia. nikitin@mail.bio.msu.ru
Summary
Nanoparticle tracking analysis (NTA) characterized novel nanovaccines. This method simultaneously measured size, aggregation, concentration, and antigen specificity of these immunogenic complexes.
Area of Science:
- Biotechnology
- Immunology
- Nanotechnology
Background:
- Biologically active nanocomplexes require characterization for vaccine development.
- Existing methods may not provide concurrent analysis of multiple properties.
Purpose of the Study:
- To apply Nanoparticle Tracking Analysis (NTA) for comprehensive characterization of a novel nanovaccine candidate.
- To concurrently assess size, aggregation, concentration, and antigenic specificity of the nanovaccine in liquid.
Main Methods:
- Utilized Nanoparticle Tracking Analysis (NTA) for real-time characterization.
- Employed thermally remodeled tobacco mosaic virus as spherical particles (SPs).
- Exposed Rubella virus tetraepitopes on the surface of SPs.
Main Results:
- NTA successfully provided concurrent data on nanovaccine size, aggregation state, and concentration.
- Antigenic specificity of the exposed tetraepitopes was assessed in liquid.
- Demonstrated the feasibility of NTA for characterizing complex nanovaccine formulations.
Conclusions:
- NTA is a powerful tool for the comprehensive characterization of nanovaccines.
- The developed nanovaccine candidate exhibits desirable properties for further development.
- Concurrent analysis of multiple parameters is crucial for nanomedicine development.
