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Gene Transfer into Older Chicken Embryos by ex ovo Electroporation
Published on: July 27, 2012
Gene transfer in developing chick embryos: in ovo electroporation
1Division of Genetics, Genomics and Development Center for Integrative Genomics, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 18, 2013
Summary
In ovo electroporation allows for quick and affordable genetic manipulation in developing chicken embryos. This method is useful for studying gene function in the neural tube and can be analyzed using techniques like qPCR.
Area of Science:
- Developmental Biology
- Molecular Genetics
Background:
- In ovo electroporation is a widely used method for studying gene function during embryonic development.
- It offers precise temporal and spatial control over genetic manipulation.
- The technique is known for its speed and cost-effectiveness.
Purpose of the Study:
- To describe the transient transfection of a construct into the neural tube of a chicken embryo using in ovo electroporation.
- To discuss modifications of the in ovo electroporation technique.
- To cover analytical methods for evaluating electroporated embryos.
Main Methods:
- In ovo electroporation for gene delivery into the chicken neural tube.
- Transient transfection of genetic constructs.
- Quantitative Polymerase Chain Reaction (qPCR) for gene expression analysis.
- Microarray analysis for comprehensive gene expression profiling.
Main Results:
- Successful transient transfection of genetic constructs into the chicken neural tube via in ovo electroporation.
- Demonstration of modifications to enhance the basic electroporation technique.
- Application of qPCR and microarray for analyzing gene expression changes in electroporated embryos.
Conclusions:
- In ovo electroporation is a versatile and efficient technique for genetic manipulation in avian embryos.
- The described methods facilitate the study of gene function during neural tube development.
- Further analysis using molecular techniques like qPCR and microarray provides insights into developmental processes.
