Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of post-simulation sonographer students' professional behaviour in the workplace.

Radiography (London, England : 1995)·2022
Same author

Factors influencing the rating of sonographer students' clinical performance.

Radiography (London, England : 1995)·2021
Same author

Evaluation of students' clinical performance post-simulation training.

Radiography (London, England : 1995)·2021
Same author

Value-added switchgrass extractives for reduction of Escherichia coli O157:H7 and Salmonella Typhimurium populations on Formica coupons.

Food microbiology·2021
Same author

Benefits and risks of adjuvant treatment with zoledronic acid in stage II/III breast cancer. 10 years follow-up of the AZURE randomized clinical trial (BIG 01/04).

Journal of bone oncology·2018
Same author

In vitro protective effects of an aqueous extract of Clitoria ternatea L. flower against hydrogen peroxide-induced cytotoxicity and UV-induced mtDNA damage in human keratinocytes.

Phytotherapy research : PTR·2018
Same journal

A native sulfur deposit in Gale crater, Mars.

Science (New York, N.Y.)·2026
Same journal

Coordinated demise of harmful algal blooms.

Science (New York, N.Y.)·2026
Same journal

Genetic effects put into context.

Science (New York, N.Y.)·2026
Same journal

Bacteria share proteins to survive antibiotics.

Science (New York, N.Y.)·2026
Same journal

Impacts shaped Earth's first continents.

Science (New York, N.Y.)·2026
Same journal

Erratum for the Report "Covalently bonded single-molecule junctions with stable and reversible photoswitched conductivity" by C. Jia <i>et al</i>.

Science (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: May 11, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

3D computational imaging with single-pixel detectors.

B Sun1, M P Edgar, R Bowman

  • 1Scottish Universities Physics Alliance, School of Physics and Astronomy, University of Glasgow, Glasgow G12 8QQ, UK. b.sun.1@research.gla.ac.uk

Science (New York, N.Y.)
|May 21, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a simplified computational imaging technique using single-pixel detectors to reconstruct 3D object shapes. The method effectively captures 3D form from multiple 2D images generated by a single projector.

More Related Videos

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Related Experiment Videos

Last Updated: May 11, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Area of Science:

  • Computational imaging
  • Optical physics
  • 3D reconstruction

Background:

  • Single-pixel detectors offer a method for spatial information retrieval.
  • Computational imaging reconstructs 2D images from projected patterns and backscattered intensity.
  • Existing 3D imaging systems can be complex and require multiple cameras.

Purpose of the Study:

  • To develop a simplified approach for 3D object reconstruction using computational imaging.
  • To demonstrate the capability of single-pixel detectors in capturing 3D form.
  • To explore the extension of this technique to nonvisible wavebands.

Main Methods:

  • Utilized multiple single-pixel detectors at various locations.
  • Projected a series of known random patterns onto the object.
  • Reconstructed 2D images from each detector, simulating different illumination directions.
  • Derived surface gradients from image shading to reconstruct the 3D object.

Main Results:

  • Successfully reconstructed the 3D form of an object using a simplified computational imaging setup.
  • Achieved 3D reconstruction from multiple 2D images generated by a single digital projector.
  • Demonstrated comparable results to stereophotogrammetric systems.

Conclusions:

  • The proposed computational imaging method provides a simplified and effective approach to 3D reconstruction.
  • The technique's reliance on single-pixel detectors and a single projector offers advantages in system design.
  • The approach shows potential for adaptation to nonvisible spectral ranges for 3D imaging.