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Enhanced Oil Recovery using a Combination of Biosurfactants

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Published on: June 3, 2022

Recombinant supercharged polypeptides restore and improve biolubrication.

Deepak H Veeregowda¹, Anke Kolbe, Henny C van der Mei

¹University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, W.J. Kolff Institute, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.

Advanced Materials (Deerfield Beach, Fla.)

|May 23, 2013

Summary

Recombinant supercharged polypeptides (SUPs) rejuvenate salivary films (SCFs) by enhancing mechanical strength and lubrication. These SUPs create a hydrated layer, improving the performance of natural salivary lubrication for extended periods.

Keywords:

biolubrication protein therapeutics scanning force microscopy sjögren's syndrome supercharged polypeptides

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Area of Science:

Biomaterials Science
Tribology
Biochemistry

Background:

Naturally occurring salivary conditioning films (SCFs) provide essential lubrication in the oral cavity.
The lubricating properties of SCFs can degrade over time, impacting oral function.
Developing methods to enhance and prolong SCF lubrication is crucial for oral health applications.

Purpose of the Study:

To develop and apply recombinant supercharged polypeptides (SUPs) for rejuvenating the lubrication of salivary conditioning films (SCFs).
To investigate the mechanism by which SUPs interact with and modify SCFs.
To evaluate the enhanced lubricating properties and mechanical stability of SCFs treated with SUPs.

Main Methods:

Recombinant expression and purification of supercharged polypeptides (SUPs) with high positive charge density.
Adsorption of SUPs onto pre-formed salivary conditioning films (SCFs).
Characterization of SUP-modified SCFs using techniques to assess mechanical strength, hydration, and mucin recruitment.
Tribological testing to evaluate sustained lubricating performance.

Main Results:

SUPs with 72 positive charges effectively adsorbed onto and rigidified SCFs.
SUPs recruited salivary mucins, promoting the formation of a stable hydrated layer.
SCFs treated with SUPs exhibited significantly higher mechanical strength compared to untreated SCFs.
The enhanced SCFs demonstrated a sustained lubricating effect for a longer duration.

Conclusions:

Recombinant supercharged polypeptides (SUPs) are effective in rejuvenating the lubricating properties of salivary conditioning films (SCFs).
SUPs enhance SCF mechanical stability and lubrication through adsorption, rigidification, and mucin recruitment.
This approach offers a promising strategy for developing improved oral lubrication solutions.