Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 11, 2026

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Fiber-optic two-photon optogenetic stimulation.

K Dhakal¹, L Gu, B Black

¹Biophysics and Physiology Laboratory, Department of Physics, University of Texas-Arlington, Texas 76019, USA.

|June 1, 2013

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Inclusive Search for Anomalous Single-Photon Production in MicroBooNE.

Physical review letters·2026

Same author

First Search for Dark Sector e^{+}e^{-} Explanations of the MiniBooNE Anomaly at MicroBooNE.

Physical review letters·2026

Same author

First Measurement of Charged-Current Muon-Neutrino-Induced K^{+} Production on Argon Using the MicroBooNE Detector.

Physical review letters·2026

Same author

Differentiation of benign and malignant complex cystic lesions of the breast: The diagnostic value of MRI.

Radiography (London, England : 1995)·2025

Same author

Recurrent macrophage activation syndrome in patients with refractory systemic lupus erythematosus treated with emapalumab: a case report.

Hong Kong medical journal = Xianggang yi xue za zhi·2025

Same author

Search for an Anomalous Production of Charged-Current ν_{e} Interactions without Visible Pions across Multiple Kinematic Observables in MicroBooNE.

Physical review letters·2025

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

This study introduces a novel fiber-optic method for two-photon optogenetic stimulation (TPOS), enabling precise cellular control without complex microscopy. This advancement improves deep-tissue optogenetics for studying cellular systems.

Area of Science:

Neuroscience
Biotechnology
Cell Biology

Background:

Optogenetic stimulation offers precise control over genetically targeted cells.
Visible light opsins face limitations in penetration depth and precision due to light scattering and absorption.
Two-photon optogenetic stimulation (TPOS) enhances spatial precision but typically requires complex microscopy setups.

Purpose of the Study:

To develop a non-scanning, fiber-optic method for TPOS.
To overcome the limitations of existing TPOS techniques that rely on microscope objectives and scanning beams.
To enable more accessible and versatile TPOS for cellular research.

Main Methods:

Utilized a multimode fiber for light delivery.
Implemented a non-scanning approach for TPOS.

More Related Videos

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

Published on: October 29, 2012

An Integrated Method for Crafting Flexible and Convenient Electrophysiological Optrodes for Multi-Region In Vivo Recording

An Integrated Method for Crafting Flexible and Convenient Electrophysiological Optrodes for Multi-Region In Vivo Recording

Published on: November 21, 2024

Related Experiment Videos

Last Updated: May 11, 2026

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

Published on: October 29, 2012

An Integrated Method for Crafting Flexible and Convenient Electrophysiological Optrodes for Multi-Region In Vivo Recording

An Integrated Method for Crafting Flexible and Convenient Electrophysiological Optrodes for Multi-Region In Vivo Recording

Published on: November 21, 2024

Demonstrated fiber-optic TPOS of cells.

Main Results:

Successfully achieved fiber-optic TPOS without complex scanning microscopy.
Enabled precise spatial modulation of genetically targeted cells via fiber optics.
Showcased a new modality for TPOS with potential for deeper tissue penetration.

Conclusions:

The developed fiber-optic TPOS method provides a simpler and potentially more versatile alternative to existing techniques.
This approach facilitates advanced cellular circuitry studies in vitro and in vivo.
Paves the way for broader adoption of TPOS in neuroscience and cell biology research.