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Related Experiment Video

Updated: May 10, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Sequential multi-molecule delivery using vortex-assisted electroporation.

Hoyoung Yun1, Soojung Claire Hur

  • 1The Rowland Institute at Harvard University, 100 Edwin H. Land Boulevard, Cambridge, MA 02142, USA.

Lab on a Chip
|June 4, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic electroporation system for precise, sequential molecule delivery into cells. The system enhances cell viability and offers real-time control for advanced cell engineering research.

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Last Updated: May 10, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
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Published on: August 7, 2014

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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Electroporation is crucial for introducing molecules into cells.
  • Existing methods face challenges in precise dosage control and cell viability.
  • On-chip systems offer potential for improved cellular manipulation.

Purpose of the Study:

  • To develop an on-chip microscale electroporation system for sequential molecular delivery.
  • To achieve precise and independent dosage controllability for multiple molecules.
  • To enhance molecular delivery efficiency and cell viability in target cells.

Main Methods:

  • Development of an on-chip microfluidic device for cell trapping and electroporation.
  • Implementation of uniform cell size trapping to improve delivery efficiency.
  • Real-time monitoring of the electroporation process for parameter adjustment.
  • Controlled delivery of membrane-impermeant molecules into human cancer cells via varied electric fields and durations.

Main Results:

  • Achieved sequential delivery of multiple molecules with precise dosage control.
  • Demonstrated enhanced molecular delivery efficiency and cell viability due to uniform cell trapping.
  • Enabled real-time monitoring and modification of electroporation parameters.
  • Successfully transferred membrane-impermeant molecules into human cancer cells.

Conclusions:

  • The developed microfluidic electroporation system offers improved cell viability and comparable gene transfection efficiency to commercial systems.
  • The system's precise control and real-time monitoring capabilities hold significant potential for expanding on-chip electroporation applications.
  • This technology advances cell engineering and molecular delivery research.