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Related Concept Videos

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Updated: May 10, 2026

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
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Bacterial immune interaction in experimental colitis.

Lin Yun Xue1, Qin Ouyang, Xuan Guang Zhou

  • 1Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.

Journal of Digestive Diseases
|June 6, 2013
PubMed
Summary
This summary is machine-generated.

5-aminosalicylic acid (5-ASA) impacts intestinal microbiota in inflammatory bowel disease (IBD) models. It promotes beneficial bacteria and reduces harmful ones, restoring microbial balance and aiding immune regulation.

Keywords:
immune systeminflammatory bowel diseaseintestinal microbiotamesalamineterminal restriction fragment length polymorphism

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Published on: September 18, 2016

Area of Science:

  • Microbiology
  • Immunology
  • Gastroenterology

Background:

  • Inflammatory bowel disease (IBD) involves complex interactions between the host immune system and intestinal microbiota.
  • Dysbiosis, an imbalance in gut bacteria, is a key feature in IBD pathogenesis.
  • Understanding the role of microbiota in IBD is crucial for developing effective therapeutic strategies.

Purpose of the Study:

  • To investigate the effects of 5-aminosalicylic acid (5-ASA) on intestinal microbiota composition and immune regulation in an IBD mouse model.
  • To explore the correlation between intestinal microbial communities and immune factors in the context of IBD.
  • To assess 5-ASA's therapeutic potential by analyzing its impact on gut homeostasis.

Main Methods:

  • Oxazolone-induced colitis model in mice.
  • Analysis of luminal and mucosal microbiota community composition using terminal restriction fragment length polymorphism (T-RFLP).
  • Measurement of key protein expressions (occludin, TLR-2, TLR-4, NF-κB p65) via immunohistochemistry and Western blot.
  • Statistical correlation analysis between microbial community structure, colitis severity, and immune factor expression.

Main Results:

  • Colitis induced significant alterations in microbiota, decreasing protective bacteria and increasing aggressive bacteria.
  • A reduction in both luminal and mucosal microbiota richness and diversity was observed in colitis, exacerbated by 5-ASA treatment.
  • Mucosal microbiota diversity showed a significant correlation with colitis severity.
  • Expressions of occludin, TLR-2, TLR-4, TNF-α, and NF-κB p65 were significantly correlated with mucosal microbiota diversity.

Conclusions:

  • Mucosal microbiota play a critical role in the pathogenesis of IBD.
  • 5-ASA treatment positively modulates the intestinal microbiota by increasing beneficial bacteria and decreasing pathogenic bacteria.
  • 5-ASA treatment helps re-establish intestinal microbial homeostasis, suggesting a therapeutic benefit in IBD.