Related Experiment Video
Updated: May 10, 2026
A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes
Published on: May 4, 2020
Rapid determination of hexapeptides by hydrophilic interaction LC-MS/MS for in vitro skin-penetration studies
Wanlong Zhou1, Perry G Wang, Oluwatosin A Ogunsola
1US FDA, Center for Food Safety & Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA.
Background:
A sensitive analytical method is needed for assessing penetration of topically applied peptides for in vitro skin-penetration studies.
Results:
A rapid hydrophilic interaction LC (HILIC)-MS/MS method for analyzing the polar peptides Ac-EEMQRR-amide and H2N-EEMQRR-amide in various skin layers and matrices has been developed and evaluated. The matrices included emulsion, receptor fluids, cotton-tipped applicators, stratum corneum tape strips, epidermis and dermis of the skin. Stable isotopically labeled analogues were used as internal standards to correct for recovery and matrix effects. A HILIC-SPE procedure was optimized to minimize significant ion suppression in the more complex matrices.
Conclusion:
This HILIC-MS/MS method is applicable to the determination of Ac-EEMQRR-amide and H2N-EEMQRR-amide in complex skin samples and other matrices generated during in vitro skin-penetration studies.
