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Production of a monoclonal antibody specific for Pou5f1/Oct4

Tatsuhiko Arakawa1, Tomohiko Yoshimi, Masayuki Azuma

  • 1Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...