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Pause, play, repeat: CDKs push RNAP II's buttons.

Miriam Sansó1, Robert P Fisher

  • 1Department of Structural and Chemical Biology; Icahn School of Medicine at Mount Sinai; New York, NY USA.

Transcription
|June 13, 2013
PubMed
Summary
This summary is machine-generated.

Cyclin-dependent kinases (CDKs) regulate both cell division and RNA polymerase II (RNAP II) transcription. These CDKs control transcription initiation, elongation, and termination, ensuring mRNA maturation and RNAP II recycling.

Keywords:
RNA polymerase IIRNA-processingTFIIHchemical geneticscyclin-dependent kinasepositive transcription elongation factor bpromoter-proximal pausingterminationtranscription

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Cyclin-dependent kinases (CDKs) are critical regulators of eukaryotic cell division.
  • Emerging evidence highlights the role of CDKs in regulating the transcription cycle of RNA polymerase II (RNAP II).
  • In metazoans, shared upstream kinases link cell cycle and transcriptional CDK networks.

Purpose of the Study:

  • To review recent advances in understanding CDK regulation of the transcription cycle.
  • To highlight the mechanisms by which CDKs influence transcription initiation, elongation, and termination.
  • To discuss the potential coupling of transcription start and end events by CDK activity.

Main Methods:

  • Chemical-genetic analyses to identify CDK substrates and their roles.
  • Review of existing literature on CDK function in transcription.
  • Analysis of phosphorylation events on the RNAP II C-terminal domain (CTD).

Main Results:

  • CDKs and their substrates control early (initiation to elongation) and late (3' end formation, termination) transcription events.
  • CDK activity may couple transcription start and end sites, ensuring mRNA processing fidelity and RNAP II recycling.
  • Transcriptional CDKs act sequentially on multiple substrates, influencing gene expression through various mechanisms.

Conclusions:

  • CDK-mediated phosphorylation of RNAP II CTD impacts transcription and co-transcriptional mRNA processing (capping, splicing, 3' end maturation).
  • CDKs may directly regulate RNA processing and histone modification machinery.
  • Further research is needed to address emerging challenges in transcription cycle regulation by CDKs.