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Genomic and structural insights into TATA-Binding protein from cestodes.

Abraham Landa¹, Elisa Heredia-Gómez^1,2, Cristina González-Kulikova^1,3

¹Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, México.

|February 27, 2026

Summary

Researchers studied TATA-binding protein (TBP) paralogs in parasitic flatworms. Findings reveal conserved structures and TATA-box binding, suggesting distinct roles for TBP1 and TBP2 in gene regulation.

Keywords:

Cestodes DNA-protein interaction TFIID electrostatic surface potential homology modeling transcription factor

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Area of Science:

Molecular Biology
Parasitology
Genomics

Background:

TATA-binding protein (TBP) is crucial for eukaryotic transcription.
Limited knowledge exists on TBP paralogs in parasitic flatworms.

Purpose of the Study:

To analyze the structure, evolution, and function of TBP1 and TBP2 paralogs in medically and veterinarily relevant cestodes.
To understand transcriptional control mechanisms in parasitic flatworms.

Main Methods:

Comparative genomic analysis of TBP paralogs.
Multiple sequence alignment and phylogenetic analysis.
Homology modeling, electrostatic potential mapping, protein-DNA docking, and molecular dynamics simulations.

Main Results:

Both TBP1 and TBP2 exhibit conserved genomic organization and TBP α/β saddle structure.
TBP1 shows sequence variability, while TBP2 is more conserved, indicating different evolutionary pressures.
All cestode TBPs retain essential residues for TATA box recognition and general transcription factor interaction.
Conserved TATA-binding patterns observed, with TBP2 showing highly uniform interactions.

Conclusions:

Genomic and structural data for cestode TBPs are provided.
Paralog-specific regulatory roles for TBP1 and TBP2 are suggested.
New insights into transcriptional regulation in parasitic flatworms are offered.