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Updated: May 10, 2026
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Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
Published on: August 16, 2016
Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual
Yael Michaeli1, Tamar Shahal, Dmitry Torchinsky
1Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv, Israel. uv@post.tau.ac.il.
Chemical Communications (Cambridge, England)
|June 13, 2013
Abstract:
Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.
