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Coumarins in horse chestnut flowers: isolation and quantification by UPLC method
Marlena Dudek-Makuch1, Irena Matławska
1Department of Pharmacognosy, University of Medical Sciences, Swiecickiego 4, 60-781 Poznań, Poland. dudum@poczta.onet.pl
Acta Poloniae Pharmaceutica
|June 14, 2013
Abstract:
The coumarins: scopoletin, esculetin and fraxetin were isolated from the flowers of horse chestnut (Aesculus hippocastanum L., Hippocastanaceae) and identified by spectrophotometric methods (UV, 1H, 13C NMR, ESI-MS). Their content, determined using the Ultra Performance Liquid Chromatography (UPLC), was 0.41, 0.13 and 0.05%, respectively.
