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Updated: May 10, 2026

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids
Published on: August 10, 2016
Rapid dissolution of DNA in a novel bio-based ionic liquid with long-term structural and chemical stability:
Chandrakant Mukesh1, Dibyendu Mondal, Mukesh Sharma
1AcSIR-Central Salt & Marine Chemicals Research Institute, G. B. Marg, Bhavnagar-364002, Gujarat, India.
Abstract:
DNA from salmon testes was solubilised in two bio-based ionic liquids up to 3.5% w/w in 6 h. No structural degradation of the molecule was observed for the sample solubilised in choline-indole-3-acetate (chol-IAA). However, the molecule was found to be denatured in choline-indole-3-butyrate (chol-IBA). The structural and chemical stability of the DNA molecules after six months of storage in the former was established. Further recyclability of the ionic liquid with very high yield (90-95%) for consecutive reuse in the redissolution of DNA was demonstrated.
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