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Updated: May 10, 2026

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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
Published on: October 28, 2014
[Kinetic parameters of calcium binding to tissue structures in human acute hypercalcaemia]
Fiziologiia Cheloveka
|June 25, 2013
Summary
This study analyzed calcium binding kinetics in healthy volunteers, revealing distinct binding patterns and cooperativity. Findings may inform diagnostic tests for tissue calcium disorders.
Area of Science:
- Biochemistry
- Physiology
- Pharmacokinetics
Context:
- Investigated calcium binding kinetics in human tissue structures.
- Utilized acute hypercalcemia induced by intravenous calcium gluconate infusion.
- Analyzed data from 19 healthy volunteers (13 men, 6 women, aged 33 ± 6.5 years).
Purpose:
- To determine the kinetic parameters of calcium binding to tissue structures.
- To analyze calcium binding isotherms using Langmuir and Scatchard models.
- To identify potential cooperativity and distinct binding segments.
Summary:
- Observed positive cooperativity in calcium binding across all volunteers.
- Identified two distinct binding segments with similar kinetic parameters (beta, K(a), n).
- Established four unique calcium binding curves in healthy individuals.
Impact:
- Characterized calcium binding properties in healthy subjects.
- Highlights variations in cooperativity thresholds and binding parameters.
- Provides a foundation for developing diagnostic tests for tissue calcium disorders.
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