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Synthesis of mesoporous multiwall ZnO nanotubes by replicating silk and application for enzymatic biosensor
Minggang Zhao1, Zhenglong Li, Ziqi Han
1Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
Abstract:
A facial biotemplate-directed synthetic route for fabricating mesoporous multiwall ZnO nanotubes (ZnO-MMNTs) was proposed. Owing to the mesoporous multiwall based matrix, one dimensional (1D) tubes based channels and high isoelectric point (IEP), the prepared ZnO-MMNTs are wonderful platform to immobilize glucose oxidase (GOx) for glucose biosensing. Scale-adaptive cells are constructed to hold enzymes molecules, maintain enzymatic activity and keep stability. The prepared enzymatic electrode (chitosan/GOx/ZnO/Au) exhibits high sensitivity (47.2 μA mM(-1)cm(-2)), very fast response (<2s), quite low KM(app) (1.09 mM), excellent selectivity and stability. Therefore, ZnO-MMNTs are promising material for enzyme assembly and other biological applications.
