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Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
Published on: July 28, 2023
Cynomolgus monkey induced pluripotent stem cells established by using exogenous genes derived from the same monkey
Nobuhiro Shimozawa1, Ryoichi Ono, Manami Shimada
1Tsukuba Primate Research Center (TPRC), National Institute of Biomedical Innovation (NIBIO), 1-1 Hachimandai, Tsukuba, Ibaraki 305-0843, Japan. shimo@nibio.go.jp
Differentiation; Research in Biological Diversity
|June 25, 2013
Summary
Researchers successfully generated induced pluripotent stem (iPS) cells from cynomolgus monkey somatic cells using species-specific genes. This breakthrough provides a valuable resource for biomedical research and drug development without immune complications.
Area of Science:
- Stem Cell Biology
- Primate Research
- Reprogramming Technology
Background:
- Induced pluripotent stem (iPS) cells share characteristics with embryonic stem (ES) cells.
- Cynomolgus monkeys are crucial models for biomedical research.
- Using xenogeneic genes in reprogramming can trigger immune responses.
Purpose of the Study:
- To establish cynomolgus monkey iPS cells using autologous genes.
- To create a valuable research resource for drug safety and efficacy studies.
- To avoid complications associated with xenogeneic gene transfer.
Main Methods:
- Infection of fetal liver and newborn skin cells with retroviral vectors.
- Delivery of cynomolgus monkey POU5F1, SOX2, KLF4, and c-MYC cDNAs.
- Culture and characterization of resulting cell lines.
Main Results:
- Formation of stable, flat colonies with large nuclei, resembling primate ES cells.
- Established cell lines exhibited normal chromosome numbers and expressed pluripotency markers.
- Generated iPS cell lines successfully formed teratomas, confirming pluripotency.
Conclusions:
- Successfully derived induced pluripotent stem cells from cynomolgus monkey somatic cells using allogeneic transgenes.
- This marks the first report of cynomolgus monkey iPS cells generated with species-specific genes.
- The method avoids additional transgenes, potentially aiding reprogramming mechanism studies.
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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
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