Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Myocyte-specific overexpressing HDAC4 promotes myocardial ischemia/reperfusion injury.

Molecular medicine (Cambridge, Mass.)·2018
Same author

MicroRNA-125b Prevents Cardiac Dysfunction in Polymicrobial Sepsis by Targeting TRAF6-Mediated Nuclear Factor κB Activation and p53-Mediated Apoptotic Signaling.

The Journal of infectious diseases·2016
Same author

Attenuation of Cardiac Dysfunction in Polymicrobial Sepsis by MicroRNA-146a Is Mediated via Targeting of IRAK1 and TRAF6 Expression.

Journal of immunology (Baltimore, Md. : 1950)·2015
Same author

Poly (I:C) therapy decreases cerebral ischaemia/reperfusion injury via TLR3-mediated prevention of Fas/FADD interaction.

Journal of cellular and molecular medicine·2014
Same author

Toll-like receptor 4 plays a central role in cardiac dysfunction during trauma hemorrhage shock.

Shock (Augusta, Ga.)·2014
Same author

Cellular cardiomyoplasty: its past, present, and future.

Methods in molecular biology (Clifton, N.J.)·2013
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Isolating Stem Cells from Soft Musculoskeletal Tissues
07:49

Isolating Stem Cells from Soft Musculoskeletal Tissues

Published on: July 5, 2010

Skeletal muscle stem cells.

Grace W Kao1, Elizabeth K Lamb, Race L Kao

  • 1Department of Surgery, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 29, 2013
PubMed
Summary
This summary is machine-generated.

Skeletal muscle satellite cells, also known as myoblasts, are key stem cells for muscle repair. This chapter details their isolation, culture, and identification for potential therapeutic applications like cellular cardiomyoplasty.

More Related Videos

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles
11:35

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles

Published on: December 9, 2022

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
07:18

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Published on: April 19, 2018

Related Experiment Videos

Last Updated: May 10, 2026

Isolating Stem Cells from Soft Musculoskeletal Tissues
07:49

Isolating Stem Cells from Soft Musculoskeletal Tissues

Published on: July 5, 2010

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles
11:35

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles

Published on: December 9, 2022

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
07:18

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Published on: April 19, 2018

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Muscle Physiology

Background:

  • Skeletal muscle satellite cells (myoblasts) are the primary stem cells responsible for muscle growth, maintenance, and repair.
  • These cells were among the first stem cells utilized for cellular cardiomyoplasty over two decades ago.
  • Understanding satellite cell biology is crucial for advancing regenerative therapies.

Purpose of the Study:

  • To provide a detailed description of the methods for isolating, culturing, labeling, and identifying skeletal muscle satellite cells.
  • To serve as a foundational guide for researchers working with these specific stem cells.
  • To complement previous work summarizing the implantation and outcomes of cellular cardiomyoplasty using satellite cells.

Main Methods:

  • Detailed protocols for the isolation of skeletal muscle satellite cells.
  • Established techniques for in vitro culture and expansion of myoblasts.
  • Methods for cell labeling and precise identification of satellite cell populations.

Main Results:

  • The chapter provides a comprehensive overview of the technical procedures required for satellite cell manipulation.
  • It establishes a standardized approach for preparing satellite cells for experimental and potentially clinical use.
  • The described methods ensure the purity and viability of satellite cells for subsequent applications.

Conclusions:

  • The detailed methodologies presented are essential for the successful application of skeletal muscle satellite cells in research and therapy.
  • Mastering these techniques is fundamental for advancing cellular cardiomyoplasty and other regenerative strategies.
  • This work lays the groundwork for further investigation into the therapeutic potential of myoblasts.