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Related Concept Videos

RNA Interference01:23

RNA Interference

24.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Related Experiment Video

Updated: Apr 28, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

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Bioengineered nanoparticles for siRNA delivery.

Kristen L Kozielski1, Stephany Y Tzeng, Jordan J Green

  • 1Department of Biomedical Engineering, The Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA; Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD, USA.

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
|July 4, 2013
PubMed
Summary
This summary is machine-generated.

Short interfering RNA (siRNA) enables gene function studies via RNA interference (RNAi). Developing safe and effective siRNA delivery systems using biomaterials and nanoparticles is crucial for therapeutic applications.

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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanomedicine

Background:

  • Short interfering RNA (siRNA) is a key tool for understanding gene function through RNA interference (RNAi).
  • Effective delivery of siRNA into target cells remains a significant challenge for both research and therapeutic applications.
  • Existing delivery strategies often adapt methods from DNA delivery, despite inherent differences in molecular barriers.

Purpose of the Study:

  • To review essential properties of biomaterials and nanoparticles for successful nucleic acid delivery.
  • To highlight advancements and challenges in siRNA delivery using various bioengineered nanomaterials.
  • To provide insights into the development of novel siRNA delivery platforms.

Main Methods:

  • Literature review of biomaterials and nanoparticle-based siRNA delivery systems.
  • Analysis of different classes of nanoparticles, including lipid-based, polymeric, inorganic, and RNA-based NPs.
  • Discussion of general principles for effective nucleic acid delivery.

Main Results:

  • Biomaterials and nanoparticles offer promising solutions for overcoming siRNA delivery barriers.
  • Various nanoparticle formulations (lipid, polymeric, inorganic, RNA-based) have shown varying degrees of success.
  • Understanding material properties is critical for optimizing siRNA encapsulation and cellular uptake.

Conclusions:

  • Advancements in biomaterials and nanoparticle engineering are vital for realizing the therapeutic potential of siRNA.
  • Continued research is needed to refine existing delivery systems and develop new ones for safe and efficient in vitro and in vivo applications.
  • Successful siRNA delivery platforms require careful consideration of material characteristics and biological interactions.