Multicommutated flow analysis system for determination of creatinine in physiological fluids by Jaffe method

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Clinical Diagnostics

Background:

• Photometric determination of creatinine is crucial for clinical diagnostics.
• Existing methods may face limitations in sensitivity, throughput, or sample handling.
• The Jaffe protocol is a widely used method for creatinine measurement.

Purpose of the Study:

• To develop a novel paired emitter detector diode (PEDD) detector for enhanced photometric creatinine determination.
• To integrate the PEDD detector into a multicommutated flow analysis (MCFA) system for automated analysis.
• To evaluate the performance of the developed system for creatinine measurement in real biological samples.

Main Methods:

• Development of a compact flow-through cell with integrated 505 nm LED emitter and 525 nm LED detector.
• Construction of a fully mechanized MCFA system controlled by a microprocessor.
• Application of a multi-point fixed-time kinetic measurement procedure.

Main Results:

• The developed PEDD-based MCFA system achieved submillimolar creatinine determination with a parts per million (ppm) detection limit.
• The system demonstrated a sample throughput of 15-40 samples per hour.
• Successful application for creatinine determination in human urine and serum samples, unaffected by sample viscosity.

Conclusions:

• The developed PEDD-based MCFA system offers a sensitive, efficient, and robust platform for photometric creatinine analysis.
• This automated system provides accurate results for real-world clinical samples.
• The system's design addresses challenges related to sample viscosity, enhancing its applicability.