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A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis
Jonathan Gisin1, Alexander Schneider, Bettina Nägele
1Department of Biology, Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany.
Abstract:
We report a salvage pathway in Gram-negative bacteria that bypasses de novo biosynthesis of UDP N-acetylmuramic acid (UDP-MurNAc), the first committed peptidoglycan precursor, and thus provides a rationale for intrinsic fosfomycin resistance. The anomeric sugar kinase AmgK and the MurNAc α-1-phosphate uridylyl transferase MurU, defining this new cell wall sugar-recycling route in Pseudomonas putida, were characterized and engineered into Escherichia coli, channeling external MurNAc directly to peptidoglycan biosynthesis.
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