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Updated: May 9, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Recent advances in the heterologous expression of microbial natural product biosynthetic pathways
Sarah E Ongley1, Xiaoying Bian, Brett A Neilan
1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia.
Abstract:
The heterologous expression of microbial natural product biosynthetic pathways coupled with advanced DNA engineering enables optimisation of product yields, functional elucidation of cryptic gene clusters, and generation of novel derivatives. This review summarises the recent advances in cloning and maintenance of natural product biosynthetic gene clusters for heterologous expression and the efforts fundamental for discovering novel natural products in the post-genomics era, with a focus on polyketide synthases (PKSs) and non-ribosomal polypeptide synthetases (NRPS).
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