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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos
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Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Published on: December 22, 2017

Optimal single-embryo mass spectrometry fingerprinting.

Alessandra Tata1, Mateus J Sudano, Vanessa G Santos

  • 1ThoMSon Mass Spectrometry Laboratory, University of Campinas, UNICAMP, Campinas, SP, Brazil. alessandratata1@gmail.com

Journal of Mass Spectrometry : JMS
|July 9, 2013
PubMed
Summary

This study presents an enhanced Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) method for analyzing lipids in single pre-implantation embryos. This advanced technique improves lipid profiling for reproductive biology and biotechnology research.

Keywords:
binary matrixembryofingerprintingmatrix-assisted laser desorption-mass spectrometry (MALDI-MS)phospholipids

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Published on: September 20, 2016

Area of Science:

  • Reproductive Biology
  • Analytical Chemistry
  • Lipidomics

Background:

  • Lipids are crucial for pre-implantation embryo viability, cryopreservation, and implantation.
  • Analyzing lipids in individual embryos is analytically challenging due to the minute quantities present.

Purpose of the Study:

  • To introduce an improved and comprehensive MALDI-MS lipid fingerprinting approach for single embryo analysis.
  • To optimize sample storage and handling conditions for MALDI-MS analysis of bovine embryos.
  • To enhance the molecular-level lipid information obtained from individual embryos.

Main Methods:

  • Utilized Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for lipid profiling.
  • Employed a binary matrix to improve detection of various phospholipid classes.
  • Performed analysis in both positive and negative ion modes (MALDI(±)-MS).
  • Tested optimal sample storage and handling protocols using bovine embryos.

Main Results:

  • The improved MALDI-MS approach significantly increased the lipid information obtained from single embryos.
  • Successfully detected phosphatidylcholines, sphingomyelins, phosphatidylserines, phosphatidylinositols, and phosphoethanolamines.
  • Established optimal conditions for sample handling and storage prior to MALDI-MS analysis.
  • Demonstrated the capability to monitor changes in membrane lipid composition during early embryonic development.

Conclusions:

  • The developed MALDI-MS protocol enables comprehensive lipidomic monitoring of single intact embryos.
  • This technique offers potential applications in human and animal reproduction, cell development, and stem cell research.
  • Provides a sensitive screening method for reproductive biology and biotechnology studies.