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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Positron Emission Tomography01:29

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
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Updated: May 9, 2026

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Compressed sensing electron tomography.

Rowan Leary1, Zineb Saghi, Paul A Midgley

  • 1Department of Materials Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge CB2 3QZ, UK. rkl26@cam.ac.uk

Ultramicroscopy
|July 10, 2013
PubMed
Summary
This summary is machine-generated.

Compressed sensing (CS) allows reconstructing signals from fewer measurements. Applying CS to electron tomography (CS-ET) significantly reduces artifacts and enables robust 3D imaging from limited data.

Keywords:
3D image reconstructionCompressed sensingCompressive samplingElectron tomographySparsity

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Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
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Last Updated: May 9, 2026

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Published on: July 12, 2022

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

Area of Science:

  • Physics
  • Mathematics
  • Materials Science

Background:

  • Compressed sensing (CS) is a mathematical framework for signal reconstruction.
  • CS theory shows sparse signals can be recovered from limited measurements.
  • Conventional electron tomography (ET) often requires numerous projections, leading to artifacts.

Purpose of the Study:

  • To apply compressed sensing (CS) principles to electron tomography (ET) reconstruction.
  • To demonstrate the effectiveness of CS-ET in reducing reconstruction artifacts.
  • To show robust 3D reconstruction is possible with significantly fewer projections.

Main Methods:

  • Implementation of compressed sensing algorithms within the electron tomography workflow.
  • Reconstruction of 3D electron tomography datasets using the CS-ET approach.
  • Comparative analysis of CS-ET reconstructions against conventional ET methods.

Main Results:

  • Marked reduction in artifacts like streaking and boundary blurring in CS-ET reconstructions.
  • Demonstration of robust 3D reconstruction from substantially fewer projections than conventionally required.
  • Improved quantitative analysis and novel 3D imaging capabilities from limited data.

Conclusions:

  • Compressed sensing significantly enhances electron tomography reconstruction quality.
  • CS-ET enables high-fidelity 3D imaging with reduced data acquisition.
  • This approach opens possibilities for advanced quantitative analysis and studies with limited datasets.