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Updated: May 9, 2026
10:33
Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor
Published on: December 7, 2015
Phenotypic CD8+ T cell diversification occurs before, during, and after the first T cell division
Fabrice Lemaître1, Hélène D Moreau, Laura Vedele
1Dynamics of Immune Responses Unit, Institut Pasteur, 75015 Paris, France.
Journal of Immunology (Baltimore, Md. : 1950)
|July 10, 2013
Summary
T cell diversity arises from both distinct clones and within individual clones. This diversification process begins before clonal expansion and continues through cell divisions.
Area of Science:
- Immunology
- Cell Biology
- T cell biology
Background:
- Effector T cell responses are characterized by cellular heterogeneity.
- The origins of this T cell diversity (programmed vs. stochastic) remain unclear.
Purpose of the Study:
- To investigate the origins and timing of T cell diversification.
- To differentiate between interclonal and intraclonal heterogeneity in T cell populations.
Main Methods:
- Utilized a sensitive in vitro assay to analyze single CD8(+) T cell progeny.
- Physically isolated daughter cells from the first T cell division.
- Developed an in vivo limiting dilution assay to assess T cell progenies post-immunization.
Main Results:
- Observed significant heterogeneity between distinct T cell clones (interclonal).
- Detected heterogeneity within individual T cell progenies (intraclonal).
- Demonstrated that paired daughter cells contribute to intraclonal diversification.
Conclusions:
- T cell diversification is a continuous process, not solely determined by clonal origin.
- Diversification is initiated prior to clonal expansion and amplified during cell divisions.
- Both interclonal and intraclonal diversification occur simultaneously in vivo.
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