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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: May 9, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Focusing and scanning microscopy with propagating surface plasmons.

B Gjonaj1, J Aulbach, P M Johnson

  • 1FOM-Institute for Atomic and Molecular Physics AMOLF, Science Park 104, 1098 XG Amsterdam, Netherlands.

Physical Review Letters
|July 16, 2013
PubMed
Summary
This summary is machine-generated.

A new surface plasmon polariton (SPP) microscope images beyond the optical diffraction limit. Using a plasmonic lens, it achieves 10 nm scanning resolution for advanced microscopy applications.

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Last Updated: May 9, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
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Published on: July 21, 2018

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
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Area of Science:

  • Optics and Photonics
  • Nanotechnology
  • Microscopy

Background:

  • Conventional optical microscopy is limited by the diffraction limit.
  • Super-resolution techniques are crucial for detailed nanoscale imaging.

Purpose of the Study:

  • To develop a novel microscope capable of imaging beyond the optical diffraction limit.
  • To demonstrate sub-10 nm resolution using surface plasmon polaritons.

Main Methods:

  • Implementation of a plasmonic lens generated via phase-structured illumination.
  • Utilizing a metallic nanostructure with alternating hole gratings and bare metal arenas.
  • Scanning the focused surface plasmon polaritons (SPPs) with 10 nm steps.

Main Results:

  • Achieved imaging resolution below the optical diffraction limit.
  • Demonstrated precise scanning of the focused SPPs.
  • Established resolution dependence on the scanning SPP focus size.

Conclusions:

  • The novel SPP microscope offers unprecedented sub-diffraction imaging capabilities.
  • Potential applications in biomedical imaging, surface biology, and functionalization chemistry.