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One-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection of three rice

Sang-Yun Cho1, Rae-Dong Jeong, Young-Nam Yoon

  • 1National Institute of Crop Science, RDA, Suwon, Republic of Korea.

Journal of Virological Methods
|July 16, 2013
PubMed

Keywords:
One-step multiplex RT-PCRRice black-streaked dwarf virusRice dwarf virusRice stripe virusSimultaneous detection

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...