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Related Experiment Video

Updated: May 9, 2026

Capturing Chromosome Conformation Across Length Scales

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

LCAT DNA shearing.

Yuka Okabe¹, Abraham P Lee

¹1Department of Biomedical Engineering, University of California-Irvine, Irvine, CA, USA.

Journal of Laboratory Automation

|July 16, 2013

Summary

This summary is machine-generated.

Researchers developed a new DNA fragmentation method using lateral cavity acoustic transducers (LCATs). This technique offers a simple, cost-effective, and low-energy approach for DNA processing on microfluidic platforms.

Keywords:

DNA shearing LCAT fragmentation ultrasound

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Last Updated: May 9, 2026

Capturing Chromosome Conformation Across Length Scales

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Published on: January 20, 2023

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

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Published on: October 5, 2018

Area of Science:

Biotechnology
Molecular Biology
Microfluidics

Background:

DNA fragmentation is a crucial step in various molecular biology applications.
Existing methods often require complex procedures, purification, or specialized equipment.

Purpose of the Study:

To introduce a novel, simplified method for DNA fragmentation.
To demonstrate the efficacy of lateral cavity acoustic transducers (LCATs) for DNA fragmentation.

Main Methods:

DNA solution was placed in a microfluidic device equipped with LCATs.
LCATs generated microstreaming to induce DNA fragmentation.
No DNA purification or downstream processing was required.

Main Results:

The LCAT-based method successfully fragmented DNA in solution.
The process was efficient, requiring minimal sample volume (<10 µL).
The method is low-cost, low-energy, and requires only a piezoelectric transducer.

Conclusions:

LCATs provide an accessible and efficient tool for DNA fragmentation.
This method is suitable for integration into automated microfluidic systems.
The technique simplifies DNA processing, reducing complexity and cost.