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Related Experiment Video

Updated: May 9, 2026

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03^YEMK

Published on: December 19, 2019

Detecting adenosine triphosphate in the pericellular space.

Simonetta Falzoni¹, Giovanna Donvito, Francesco Di Virgilio

¹Department of Morphology, Surgery and Experimental Medicine, Section of General Pathology , University of Ferrara , Via Borsari 46, Ferrara 44121 , Italy.

Interface Focus

|July 16, 2013

Summary

Researchers developed a novel plasma membrane luciferase probe to accurately measure extracellular adenosine triphosphate (ATP) levels. This tool enables sensing ATP in various conditions, including inflammation and tumors.

Keywords:

bioluminescence cancer extracellular adenosine triphosphate inflammation luciferase

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Last Updated: May 9, 2026

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Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

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Published on: March 11, 2021

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Extracellular adenosine triphosphate (ATP) plays crucial roles in physiological and pathological processes.
Measuring extracellular ATP has been technically challenging.
Previous methods for detecting extracellular ATP were limited.

Purpose of the Study:

To engineer a reliable biosensor for quantifying extracellular ATP.
To develop a tool for monitoring ATP levels in disease models.

Main Methods:

Construction of a chimeric protein: firefly luciferase fused with folate receptor leader and anchor sequences.
Targeting and expression of the chimeric protein to the plasma membrane outer surface.
Generation of stably transfected HEK293 cell clones as ATP sensors.

Main Results:

Successfully localized a novel luciferase probe to the plasma membrane outer surface.
Developed HEK293 cell clones capable of sensing extracellular ATP concentrations.
Demonstrated the utility of the probe in detecting ATP in disease models like tumors and inflammation.

Conclusions:

The plasma membrane luciferase probe provides a robust method for measuring extracellular ATP.
This engineered biosensor facilitates in vitro and in vivo monitoring of ATP levels.
The tool has potential applications in studying ATP's role in various diseases.