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Updated: May 9, 2026
06:19
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
[Oxidative stress in chronic hepatitis C patients]
Jing Zhao1, Yu-chen Fan, Feng Zhang
1Department of Hepatology, Qilu Hospital of Shangdong University, Jiang, China.
Summary
Chronic hepatitis C (CHC) patients exhibit increased oxidative stress, with markers worsening in those with elevated alanine aminotransferase (ALT) levels. This highlights the role of oxidative damage in CHC progression.
Area of Science:
- Biochemistry
- Hepatology
- Oxidative Stress Research
Context:
- Chronic hepatitis C (CHC) is a significant global health concern.
- Understanding the biochemical alterations in CHC is crucial for managing the disease.
- Oxidative stress plays a role in the pathogenesis of various chronic liver diseases.
Purpose:
- To investigate the status of oxidative stress in patients with chronic hepatitis C.
- To compare oxidative stress markers between CHC patients with normal and elevated alanine aminotransferase (ALT) levels.
- To explore correlations between liver enzymes, viral load, and oxidative stress markers in CHC.
Summary:
- Serum levels of xanthine oxidase (XOD), malondialdehyde (MDA), glutathione S-transferase (GST), and glutathione reductase (GR) were elevated in CHC patients compared to controls.
- Conversely, glutathione (GSH), glutathione peroxidase (GSH-Px), and vitamin C (Vc) levels were decreased in CHC patients.
- Elevated ALT levels in CHC patients were associated with more severe oxidative stress, indicated by higher XOD, MDA, GSSG, GST, and GR, and lower GSH, GSH-Px, and Vc.
Impact:
- This study quantifies oxidative stress in CHC, providing biochemical evidence of its contribution to liver damage.
- Findings suggest that elevated ALT levels serve as an indicator of heightened oxidative stress in CHC patients.
- The results may inform the development of targeted therapies aimed at mitigating oxidative stress in CHC management.
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