Using PCR-RFLP technology to teach single nucleotide polymorphism for undergraduates

Area of Science:

• Genetics
• Molecular Biology
• Cancer Research

Background:

• Aberrant gene expression of peroxiredoxin-6 (Prdx6) is observed in various cancers.
• The association between Prdx6 genetic polymorphism and cancer onset warrants investigation due to its role in cancer cells.

Purpose of the Study:

• To develop and implement a laboratory course for undergraduate students to learn about single nucleotide polymorphisms (SNPs).
• To teach students about the relationship between Prdx6 gene variations and cancer onset through practical molecular biology techniques.

Main Methods:

• Utilized the Prdx6 gene's rs4382766 polymorphism, which contains an SspI restriction site, as a target.
• Integrated techniques including database retrieval, genomic DNA isolation, Polymerase Chain Reaction (PCR), and restriction enzyme assay (PCR-RFLP).

Main Results:

• Successfully developed a serial experiment for teaching molecular biology concepts and techniques.
• Students gained a comprehensive understanding of molecular biology and the interconnectedness of experimental procedures.

Conclusions:

• The developed PCR-RFLP lab course effectively teaches undergraduate students about SNPs and molecular biology.
• The hands-on approach increased student engagement and enthusiasm compared to previous curricula.