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Published on: November 21, 2023
Cyclic enzymatic amplification method (CEAM) based on exonuclease III for highly sensitive bioanalysis.
Liang Cui1, Guoliang Ke, Xiaoyan Lin
1State Key Laboratory of Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Science, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
Cyclic enzymatic amplification method (CEAM) overcomes traditional nucleic acid probe limitations by enabling sensitive detection of DNA, proteins, and small molecules. This review details CEAM
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- Nucleic acid molecular probes (NAMPs) are crucial for sensing diverse analytes.
- Traditional NAMPs suffer from limited sensitivity due to a 1:1 binding ratio.
Purpose of the Study:
- To review the cyclic enzymatic amplification method (CEAM) for enhanced analyte detection.
- To highlight CEAM's advantages in sensitivity, selectivity, and versatility.
Main Methods:
- Introduction of the working principle of CEAM based on exonuclease III.
- Discussion of CEAM applications with various output signals.
- Detailed experimental designs using displacing probes and linear molecular beacons (LMBs).
Main Results:
- CEAM significantly enhances signal amplification compared to traditional methods.
- Demonstrated successful application in optical and electrical detection of DNA, proteins, and small molecules.
- CEAM offers excellent sensitivity, selectivity, versatility, and simplicity.
Conclusions:
- CEAM represents a powerful strategy to overcome the limitations of traditional NAMPs.
- The method provides a versatile platform for sensitive and selective detection across various fields.
- Further exploration of CEAM designs and applications is warranted.
