Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Topographical control of cell behaviour: II. Multiple grooved substrata.

P Clark1, P Connolly, A S Curtis

  • 1Department of Cell Biology, University of Glasgow, Scotland, UK.

Development (Cambridge, England)
|April 1, 1990
PubMed
Summary

Cell alignment on grooved surfaces depends on topography. Deeper grooves and specific cell types, like BHK and MDCK cells, show greater alignment, influenced by cell-cell interactions and cytoskeletal organization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Nicotine Delivery Systems.

Advances in dental research·2019
Same author

Reptarenaviruses in apparently healthy snakes in an Australian zoological collection.

Australian veterinary journal·2019
Same author

The burden of musculoskeletal disorders in Mexico at national and state level, 1990-2016: estimates from the global burden of disease study 2016.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2018
Same author

Correction to: Global dietary calcium intake among adults: a systematic review.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2018
Same author

Health-related quality of life during the first year after a hip fracture: results of the Mexican arm of the International Cost and Utility Related to Osteoporotic Fractures Study (MexICUROS).

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2018
Same author

FRAX-based intervention and assessment thresholds in seven Latin American countries.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2017

Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Cellular contact guidance is crucial for development.
  • Substratum topography influences cell behavior.
  • Miniaturization techniques enable precise surface fabrication.

Purpose of the Study:

  • Investigate topographical guidance of BHK, MDCK, and chick embryo cerebral neurons.
  • Determine the effect of groove dimensions (repeat spacing and depth) on cell alignment.
  • Analyze how cellular properties affect susceptibility to topographical cues.

Main Methods:

  • Fabricated grooved substrata using electronics miniaturization.
  • Examined cell alignment on substrata with varying groove dimensions (4-24 microns repeat, 0.2-1.9 microns depth).

Related Experiment Videos

  • Utilized scanning electron microscopy for detailed cell-surface interaction analysis.

    • Main Results:

    • Cell alignment increased with groove depth, showing inverse proportionality to repeat spacing.
    • BHK and MDCK cells exhibited differential responses to grooved substrata.
    • MDCK cell response varied based on isolation or epithelial island context.
    • Combined topographical cues yielded greater guidance than predicted from single cues.

    Conclusions:

    • Groove depth is a more significant factor than spacing for cell alignment.
    • Cellular properties like cytoskeletal organization and cell adhesion mediate topographical responses.
    • Substratum topography is a critical cue in developmental processes, with cell-cell interactions modulating responses.